Bacillus Globigii Endospores Reduction

Jamie Balarashti, Zach Conley
Aerosol Research and Engineering Laboratories, Kansas
Full Report


To evaluate the efficacy of the Defend 400 at reducing aerosolized Bacillus Globigii bacterial endospores a commonly used surrogate for Bacillus Anthraxis.


Bacillus Globigii was aerosolized into a 16m3 sealed environmental bioaerosol chamber containing the Defend 400.

AGI impingers were used to sample the chamber bioaerosol concentrations. All impinger samples were serially diluted, plated and enumerated in triplicate to yield viable bioaerosol concentration at each sampling point and time. Samples were taken at 0, 15, 30, 45 and 60 minutes in order to quantify the reduction speed and capabilities of the Defend 400. Chamber control trial data was subtracted from the Defend 400 trial data to yield net LOG reduction in the chamber for the bioaerosol challenges.

This study was conducted in compliance with FDA Good Laboratory Practices (GLP) as defined in 21 CFR, Part 58.

Summary of Results

The Defend 400 achieved a net LOG reduction of Bacillus Globigii endospores after 45 minutes of 4.55 which is equivalent to a net percent reduction of 99.9969%.

Defend 400 achieved 99.99% reduction of Bacillus Globigii endospores

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